Cancer and Translational Research Lab D.Y.Patil Institute of Biotechnology and Bioinformatics
Title: To identify modified nucleoside biomarkers in the nail clippings of oral cancer patients by using the VTGE tool.
Investigated a study on non-invasive oral cancer detection, focusing on using nail clippings as a novel biological material for early diagnosis of Oral Squamous Cell Carcinoma (OSCC).
Strengthened the foundation of cancer research by systemizing the collection and preparation of biological samples, adhering to ethical guidelines, and ensuring the integrity of samples for accurate analysis.
Implemented integrated approaches to fractionate nucleoside metabolite biomarkers, utilizing vertical tube gel electrophoresis for precise analysis
Uncovered a potential set of biomarkers in the modified nucleoside levels within nail clippings of oral cancer patients, setting a precedent for non-invasive diagnostic methods.
Conducted qualitative and quantitative assessments of modified nucleosides using UV-visible spectroscopy and Liquid Chromatography-Mass Spectrometry (LC-MS) contributing to a profound increase in the accuracy of early detection.
Identified elevated levels of specific nucleosides such as inosine, succinyl adenosine, 5,6-dihydrouridine, deoxyinosine, and 5-methylcytidine in oral cancer patients compared to healthy subjects.
